Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants

Update on Plastid Proteostatis in Transplastomic Plants Plastid Proteostasis and Heterologous Protein Accumulation in Transplastomic Plants

The insertion of transgenes into the plastid genome (plastome) has proved to be an effective alternative to nuclear transformation for producing stable transformants expressing heterologous proteins. Recently, methodology, characteristics, and possible fields of application of plastome transformation have been extensively reviewed (Lössl and Waheed, 2011; Scotti et al., 2012). From these report...

Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids.

We isolated an Arabidopsis thaliana cDNA whose translated product shows sequence similarity to the FtsY, a bacterial homologue of SRP receptor protein. The Arabidopsis FtsY homologue contains a typical chloroplast transit peptide. The in vitro-synthesized 37 kDa FtsY homologue was imported into chloroplasts, and the processed 32 kDa polypeptide bound peripherally on the outer surface of thylako...

Plastid proteostasis and heterologous protein accumulation in transplastomic plants.

Identification of a Chloroplast Dehydrin in Leaves of Mature Plants

Several types of proteins are known to accumulate as a result of dehydration stress in plants, and many of these are thought to serve a protective function. This includes the dehydrin family of proteins, which accumulate in cells in response to drought, low temperatures, or salinity and in embryo tissues during the maturation phase of seed development, when the seed is losing water in preparati...

Homoplasmic Stability and Cytoplasmic Inheritence of DARPin G3 Scaffold Protein in Generative and Vegetative Propagation of Transplastoic Tobacco Plants

Plastid engineering gives numerous benefits for the next generation of transgenic technology, consisting of the convenient use of transgene stacking and the production of high expression levels of recombinant proteins. Designed ankyrin repeat proteins (DARPin) are relatively small non-immunoglobulin scaffold proteins that bind to their specific target with high affinity. The G3 is a type of DAR...